Isolation of a - Cellulose from Nipa ( Nypa fruticans Wurmb) Frond using Physico-Chemical Treatment

Objective: The aim of this research is to create a versatile approach produce cellulose from nipa fronds, which are sustainable resource. Methods: study focuses on determining the amount in effectiveness simplified physicochemical pretreatment, and purity isolated a-cellulose. biomass was pretreated using physical chemical methods analyzed through gravimetric analysis determine its composition, delignification efficiency at varying concentrations Sodium hydroxide (NaOH), a-cellulose (iNaC) National Renewable Energy Laboratory (NREL) protocol, ”NREL/TP-510- 42618”. data statistically One-way Analysis Variance. Findings: results showed that increasing concentration NaOH led positive responses delignification, decreased hemicellulose, increased recovery cellulose. found as increased, recovered also increased. Treatment 4 (15% NaOH) 2 (10% produced 79% w/w 77.45% a-cellulose, respectively. While highly effective breaking down biomass, statistical revealed comparable terms producing pulp frond without requiring higher NaOH. This reduction waste, making more option. Keywords: a-cellulose; Nipa Frond; Biomass; Soda Treatment; Lignocellulosic; NREL protocol